產品介紹

Biocolor 彈性蛋白檢測試劑盒
Biocolor Fastin elastin Assay 

利用染料結合的方法來定量檢測釋放到組織培養(yǎng)液或從生物材料中提取的彈性蛋白。
 
應用范圍：樣本可來源于細胞培養(yǎng)基和含細胞外基質成分的組織提取物（如彈性軟骨、主動脈、皮膚等），可檢測可溶性彈性蛋白原和不可溶性彈性蛋白（抽提后以可溶性彈性蛋白多肽的形式檢測）。
 
樣本要求：樣本體積5-100ul，包含5-75ug的彈性蛋白
 
實驗操作步驟

彈性蛋白的分離：用彈性蛋白沉淀劑處理樣本，使彈性蛋白沉淀下來。
彈性蛋白與染料結合：裝有沉淀的彈性蛋白管中，加染料和反應液，反應60min以上
彈性蛋白-染料復合物獲得：去掉未結合的染料
復合物染料的釋放：添加染料釋放劑，使彈性蛋白-染料復合物中的染料釋放出來
釋放出的染料檢測：繪制標準曲線，513nm或405nm處定量檢測

產品訂購

   Product Code: F2000        110 assays

   Product Code: F4000        440 assays

參考文獻

1
Allen, R. A., Wu, W., Yao, M., Dutta, D., Duan, X., Bachman, T. N., Champion, H. C., Stolz, D. B., Robertson, A. M.,
Kim, K., et al. (2014) Nerve regeneration and elastin formation within poly (glycerol sebacate)-based synthetic arterial grafts one-year post-implantation in a rat model. Biomaterials 35, 165–173.
2
Anilkumar, T. V., Vineetha, V. P., Revi, D., Muhamed, J. and Rajan, A. (2014) Biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. Journal of Biomedical Materials Research Part B:
Applied Biomaterials.
3
AUGUSTIN, C., FREI, V., PERRIER, E., HUC, A. and DAMOUR, O. AN IN VITRO SELECTION OF NEW COSMETIC ACTIVE
COMPOUNDS: FROM SCREENING TESTS ON MONOLAYERED FIBROBLAST CULTURE TO EFFICIENCY STUDY ON 3-D DERMAL
EQUIVALENT. AMMONIO LATTATO" ATTIVATO 1.
4
Baiguera, S., Del Gaudio, C., Kuevda, E., Gonfiotti, A., Bianco, A. and Macchiarini, P. (2014) Dynamic decellularization and cross-linking of rat tracheal matrix. Biomaterials 35, 6344–6350.
5
Baiguera, S., Del Gaudio, C., Lucali, E., Kuevda, E., Boieri, M., Mazzanti, B., Bianco, A. and Macchiarini, P. (2014) Electrospun gelatin scaffolds incorporating rat decellularized brain extracellular matrix for neural tissue engineering. Biomaterials 35, 1205–1214.
6
Cheheltani, R., McGoverin, C. M., Rao, J., Vorp, D. A., Kiani, M. F. and Pleshko, N. (2014) Fourier transform infrared spectroscopy to quantify collagen and elastin in an in vitro model of extracellular matrix degradation in aorta. Analyst 139, 3039–3047.
7
Chen, M.-H., Hu, C.-K., Chen, P.-R., Chen, Y.-S., Sun, J.-S. and Chen, M.-H. (2014) Dose-dependent regulation of cell proliferation and collagen degradation by estradiol on ligamentum flavum. BMC musculoskeletal disorders 15, 238.
8
Chow, M.-J., Turcotte, R., Lin, C. P. and Zhang, Y. (2014) Arterial Extracellular Matrix: A Mechanobiological Study of the Contributions and Interactions of Elastin and Collagen. Biophysical journal 106, 2684–2692.
9 Gilpin, S. E., Guyette, J. P., Gonzalez, G., Ren, X., Asara, J. M., Mathisen, D. J., Vacanti, J. P. and Ott, H. C. (2014) Perfusion decellularization of human and porcine lungs: Bringing the matrix to clinical scale. The Journal of Heart and Lung Transplantation 33, 298–308.
10
Gu, Q., Wang, B., Zhang, X.-F., Ma, Y.-P., Liu, J.-D. and Wang, X.-Z. (2014) Chronic aerobic exercise training
attenuates aortic stiffening and endothelial dysfunction through preserving aortic mitochondrial function in aged rats. Experimental gerontology 56, 37–44.
11
Gu, Q., Wang, B., Zhang, X.-F., Ma, Y.-P., Liu, J.-D. and Wang, X.-Z. (2014) Contribution of renin-angiotensin system to exercise-induced attenuation of aortic remodeling and improvement of endothelial function in spontaneously hypertensive rats. Cardiovascular Pathology.
12
Isayama, N., Matsumura, G., Sato, H., Matsuda, S. and Yamazaki, K. (2014) Histological maturation of vascular smooth muscle cells in in situ tissue-engineered vasculature. Biomaterials 35, 3589–3595.13
Jelinic, M., Leo, C.-H., Uiterweer, E. D. P., Sandow, S. L., Gooi, J. H., Wlodek, M. E., Conrad, K. P., Parkington, H., Tare, M. and Parry, L. J. (2014) Localization of relaxin receptors in arteries and veins, and region-specific increases in compliance and bradykinin-mediated relaxation after in vivo serelaxin treatment. The FASEB Journal 28, 275–287.
14
Larroque-Cardoso, P., Mucher, E., Grazide, M.-H., Josse, G., Schmitt, A.-M., Nadal-Wolbold, F., Zarkovic, K., Salvayre, R. and Nègre-Salvayre, A. (2014) 4-Hydroxynonenal impairs transforming growth factor-β1-induced elastin synthesis via epidermal growth factor receptor activation in human and murine fibroblasts. Free Radical Biology and Medicine 71,
427–436.
15
Latimer, C. A., Nelson, M., Moore, C. M. and Martin, K. E. (2014) Effect of collagen and elastin content on the burst pressure of human blood vessel seals formed with a bipolar tissue sealing system. Journal of Surgical Research 186, 73–80.
16
Liu, S., Parameswaran, H., Young, S. M. and Varisco, B. M. (2014) JNK suppresses pulmonary fibroblast elastogenesis during alveolar development. Respiratory research 15, 34.
17
Merrilees, M. J., Falk, B. A., Zuo, N., Dickinson, M. E., May, B. C. and Wight, T. N. (2014) Use of versican variant V3 and versican antisense expression to engineer cultured human skin containing increased content of insoluble elastin.Journal of tissue engineering and regenerative medicine.
18
Mizuno, M., Takebe, T., Kobayashi, S., Kimura, S., Masutani, M., Lee, S., Jo, Y. H., Lee, J. I. and Taniguchi, H. (2014) Elastic Cartilage Reconstruction by Transplantation of Cultured Hyaline Cartilage–Derived Chondrocytes. In Transplantation proceedings, pp 1217–1221, Elsevier.
19
Price, A. P., Godin, L. M., Domek, A., Cotter, T., D’Cunha, J., Taylor, D. A. and Panoskaltsis-Mortari, A. (2014)
AUTOMATED DECELLULARIZATION OF INTACT, HUMAN-SIZED LUNGS FOR TISSUE ENGINEERING. Tissue Engineering.
20
Santoso, E. G., Yoshida, K., Hirota, Y., Aizawa, M., Yoshino, O., Kishida, A., Osuga, Y., Saito, S., Ushida, T. and Furukawa, K. S. (2014) Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models. PloS one 9, e103201.
21
Sivan, U., Jayakumar, K. and Krishnan, L. K. (2014) Matrix-directed differentiation of human adipose-derived mesenchymal stem cells to dermal-like fibroblasts that produce extracellular matrix. Journal of tissue engineering and regenerative medicine.
22
Smith, K. D., Clegg, P. D., Innes, J. F. and Comerford, E. J. (2014) Elastin content is high in the canine cruciate ligament and is associated with degeneration. The Veterinary Journal 199, 169–174.
23
Tam, K., Cheyyatraviendran, S., Venugopal, J., Biswas, A., Choolani, M., Ramakrishna, S., Bongso, A. and Fong, C.-Y. (2014) A nanoscaffold impregnated with human Wharton’s Jelly stem cells or its secretions improves healing of wounds. Journal of cellular biochemistry 115, 794–803.